Journal: BMC Microbiology

Article Title: A Multilocus Sequence Typing System (MLST) reveals a high level of diversity and a genetic component to Entamoeba histolytica virulence

doi: 10.1186/1471-2180-12-151

Figure Lengend Snippet: Verification, by Sanger sequencing, of 12 polymorphic loci identified by Next Generation Sequencing (NGS) of E. histolytica genomes

Article Snippet: A presumptive diagnosis of ALA was based on clinical picture, ultrasound examination and positive serology using an E. histolytica antigen based ELISA (TechLab E . histolytica II) [ ].

Techniques: Sequencing, Next-Generation Sequencing

Journal: BMC Microbiology

Article Title: A Multilocus Sequence Typing System (MLST) reveals a high level of diversity and a genetic component to Entamoeba histolytica virulence

doi: 10.1186/1471-2180-12-151

Figure Lengend Snippet: Similarity of E. histolytica diversity in Bangladeshi and whole genome sequenced strains. Shown on the y axis (H) is the calculated heterozygosity and represents sum of the squared allele frequencies was subtracted from 1 on the x axis the loci containing the SNPs genotyped by MSLT(■ value in Bangladesh samples genotyped during this study, (□ value in the sequenced genomes described in Table ).

Article Snippet: A presumptive diagnosis of ALA was based on clinical picture, ultrasound examination and positive serology using an E. histolytica antigen based ELISA (TechLab E . histolytica II) [ ].

Techniques:

Journal: BMC Microbiology

Article Title: A Multilocus Sequence Typing System (MLST) reveals a high level of diversity and a genetic component to Entamoeba histolytica virulence

doi: 10.1186/1471-2180-12-151

Figure Lengend Snippet: Lack of consistent patterns of descent among SNP markers from Bangladeshi E. histolytica isolates suggests they segregate independently. Consensus phylogeny inferred from 100 bootstrap replicates of polymorphic SNP markers, constructed using the MEGA 5 program and the Maximum Likelihood method based on the Tamura-Nei model and using the sequences shown in Additional file : Table 8 . Branches produced in fewer than 50% of the bootstrap phylogenies were collapsed. Sequences from stool have the suffix s; culture c; monthly survey stools begin with MS or CMS, diarrheal DS or CDS, amebic liver abscess samples RUF.

Article Snippet: A presumptive diagnosis of ALA was based on clinical picture, ultrasound examination and positive serology using an E. histolytica antigen based ELISA (TechLab E . histolytica II) [ ].

Techniques: Construct, Produced

Journal: BMC Microbiology

Article Title: A Multilocus Sequence Typing System (MLST) reveals a high level of diversity and a genetic component to Entamoeba histolytica virulence

doi: 10.1186/1471-2180-12-151

Figure Lengend Snippet: Locations of informative SNPs

Article Snippet: A presumptive diagnosis of ALA was based on clinical picture, ultrasound examination and positive serology using an E. histolytica antigen based ELISA (TechLab E . histolytica II) [ ].

Techniques:

Journal: BMC Microbiology

Article Title: A Multilocus Sequence Typing System (MLST) reveals a high level of diversity and a genetic component to Entamoeba histolytica virulence

doi: 10.1186/1471-2180-12-151

Figure Lengend Snippet: Genomes sequenced by the Genomic Sequencing Center for Infectious Diseases (GSCID) and the Institute of Integrative Biology, E. histolytica Genome sequencing projects

Article Snippet: A presumptive diagnosis of ALA was based on clinical picture, ultrasound examination and positive serology using an E. histolytica antigen based ELISA (TechLab E . histolytica II) [ ].

Techniques: Genomic Sequencing, Sequencing